CaMKIV is not required for terminal differentiation and cytokine synthesis induced by LPS. Isolated CD11c⁺ splenic DCs from WT and Camk4^−/− were exposed to LPS (10 μg/mL) or left untreated (none). After 16 hours, cells were recovered and double-stained for I-A and CD86 (A). Aliquots of cells were stained for the presence of intracellular TNF-α and IL-6 (A,B).