The number of splenic mature DCs is reduced in Camk4^−/− mice. Splenocytes from normal or Camk4^−/− mice were counted and stained for CD11c and CD8. (A) Left panel: immunoblots show CaMKIV and actin expression in splenocytes isolated from 2 mice from each genotype. Middle: bar graph reports mean (± SD) of the total number of splenocytes (n = 15 mice per genotype). Right: percentage of WT and Camk4^−/− CD11c + subsets. Bars graphs show mean (± SD) representing 15 mice per genotype. *P < .01. (B) LPS-induced mature DC accumulation is impaired in Camk4^−/− mice in vivo. LPS or phosphate-buffered saline (PBS) was injected into Camk4^−/− and control WT mice. Eighteen hours later, splenocytes were isolated and triple-stained with anti-CD11b, -CD11c and -I-A antibodies. For the typical dot plot profiles, the inset values show the percentage of cells in the R6/R7 gates (CD11b^high/CD11c^high and CD11b^low/CD11c^high, respectively. FACS profile histograms show I-A expression. Inset values refer to the percentage of I-A^high cells in the R6/R7 gates. Values in parenthesis display the percentage of CD11b^high/CD11c^high/I-A^high and CD11b^low/CD11c^high/I-A^high in whole splenocytes. *P < .01.