CaMKs regulate terminal differentiation and survival of monocyte-derived dendritic cells. Immature monocyte–derived DCs were cultured untreated or stimulated with LPS- (1 μg/μL) in the presence or absence of KN93 (10 μM), a selective inhibitor of the multifunctional CaMKs. After 24 hours, cells were recovered and double-stained with anti-CD86/anti-CD83 antibodies or with Annexin V/propidium iodide (A,B top). (A) Bottom: effects of KN93 on CD83 and CD86 expression as a function of the LPS dose. Mean (± SD) represents 6 independent experiments. (B) Bottom: effects of KN93 on survival of LPS-stimulated DC (LPS, 10 μg/mL) as a function of time or KN93 dose (left or right, respectively). Viability was calculated by trypan blue exclusion. Mean (± SD) represents 6 independent experiments.*P < .01. Values in panel A represent the mean fluorescente intensity of CD86 and the percentage of CD83 positive cells. Ctr refers to profiles of unstained cells. Values in panel B represent the percentage of cells in each quadrant.