Scheme of constructs and sequence alignment of the regions encompassing the furin motif of RGM proteins. (A) ClustalW alignment (http://www.ebi.ac.uk/clustalw)¹⁴  of human RGMs, showing amino acidic conservation 5′ and 3′ of the furin motif. RSER³³²NRR³³⁵ consensus sequence is boxed. The * above the boxed motif marks arginine 335. The degrees of conservation between proteins are indicated as follows: * indicates identical residue; :, conserved substitution; and ., semiconserved substitution. (B) From top to bottom, schematic representation of HJV functional domains in wild-type HJV and localization of the studied mutation (HJV^R335Q) and chimeric variant (HJV^KDEL). SP indicates signal peptide; RGD, arginine-glycine–aspartic acid integrin-binding domain; ◇, Cys residue; and ●, Asn residue. The dotted line indicates the autoproteolytic site. The double line shows the peptide used for the antibody production.