Mature megakaryocyte number and ploidy are increased in Pf4-Cre/FAK-floxed mice. (A) Sections of mouse femora were stained with VWF and hematoxylin to identify megakaryocytes. The section shown is representative of preparations analyzed. (B) VWF-positive and morphologically recognizable megakaryocytes were counted per field of view. Numbers represent the mean (± SEM) of 10 counts taken from 3 mice in each group. ***P < .001 ≥. (C,D) In vivo ploidy analysis of CD41-positive bone marrow cells taken from WT and Pf4-Cre/FAK-floxed mice. CD41-positive ploidy peaks were gated on fluorescence on individual DNA peaks. The data shown are representative of 3 independent experiments.