Plasmin-sensitive lmw-scuPA and scFv/uPA, but not scFv/uPA-T, deplete fibrinogen from mouse plasma. (A) Concentration of fibrinogen in pooled mouse plasma treated with lmw-scuPA, scFv/uPA or scFv/uPA-T for 3 hours. (B) Concentration of fibrinogen in plasma of mice injected with scFv/uPA-T (120 μg) or the same dose of lmw-scuPA and scFv/uPA. scFv/uPA-T–injected mice showed intact plasma fibrinogen level, in contrast to lmw-scuPA– or scFv/uPA-treated mice (*P < .05, vs lmw-scuPA cohort; #P < .05, vs scFv/uPA cohort). Error bars represent SEM.