The distribution of calreticulin indices and response to high-dose melphalan. Immunohistochemical staining of baseline marrow biopsies was performed as described. (A) Distribution of calreticulin indices for patients with CR (n = 20), PR (n = 12), and NR (n = 16) shown. The difference between CR and NR groups is significant and there is a trend toward significance in the comparison of CR and PR groups. (B) qRT-PCR levels of patients with indices of 1 to 4 (n = 7) are compared with those of patients with indices 6 to 9 (n = 17; P = .09, unpaired t test). Horizontal bars are mean values.