Silencing Lyn inhibits the mTOR pathway independently of Akt in AML cells. (A) KG1 and U937 were cultured after electroporation with Lyn siRNA or control siRNA. After 72 hours, cells were processed for Western blot analysis using appropriate antibodies. Results shown are representative of 4 independent experiments. (B) Fresh AML samples (patient nos. 15, 18, 45) were cultured during 48 hours after electroporation with Lyn siRNA or control siRNA, lysed, and analyzed by Western blot using indicated antibodies.