Inhibition of SFKs affects the phosphorylation of mTOR targets in AML cells. (A) KG1 cells were cultured for 1 hour alone (control) or in the presence of rapamycin (10 nM), AG1296 (25 μM), AG490 (20 μM), and PP2 (10 μM). Cells were lysed and analyzed by Western blot with the indicated antibodies. Results shown are representative of 3 independent experiments. (B) Fresh AML cells (patient nos. 1–20) were cultured for 1 hour alone or in the presence of PP2 (10 μM), lysed, and analyzed as in panel A. Results shown are representative of the different patients analyzed. (C) Fresh AML cells (patient nos. 7, 19, 21, 42, 43, 45, 49, 53, 59) were cultured alone or in the presence of PP2 (10 μM) or LY294002 (25 μM) for 4 hours, then processed for Western blot analysis.