Effect of IVIG on the recruitment of leukocyte subsets by multichannel fluorescence intravital microscopy. (A) Leukocyte subsets were identified by injection of low doses of antibodies against CD45 (red), Gr-1 (green), and F4/80 (blue). Colored arrowheads indicate adherent leukocytes in representative venules according to their subset identification with this method. Bar, 20 μm. (B) IVIG reduces the number of CD45 + cells. ***P < .001. (C) IVIG significantly reduced the recruitment of neutrophils (CD45 + Gr-1 + F4/80−) but did not affect the recruitment of monocytes (CD45 + F4/80 +) and lymphocytes (CD45 + Gr-1−F4/80−). ***P < .001.