Effects of TNF-α and IVIG on adhesion molecule expression on Gr-1 + leukocytes by flow cytometric analyses. Blood from sickle cell mice was harvested at baseline, 70 minutes after TNF-α (IVIG not yet administered); 120 minutes after TNF-α (30 minutes after IVIG infusion); and 150 minutes after TNF-α (60 minutes after IVIG infusion). (A) E-selectin binding. ESLs were detected using a chimeric E-selectin–IgM recombinant protein. Control sample was incubated in the presence of 10 mM EDTA. (B) LFA-1 and (C) PSGL-1 expression. Controls were stained with isotype-matched IgG.