CD1d-dependent NKT cells in β2m^−/− mice with and without the Stat5b transgene. (A) Thymic sTCRβ/CD1d tetramer profiles of 18-week-old β2m^−/− mice with and without the Stat5b transgene. Studies of age-matched C57BL/6 mice, with and without the Stat5b transgene, are shown for comparison. Both the Stat5b TG mice on the WT background and Stat5b TG β2m^−/− mice had evidence of lymphoma (enlarged thymus, increased cellularity, and characteristic CD3/FSC and CD4/CD8 profiles; data not shown). Percentage of gated cells is indicated with each profile, and the total cellularity of the thymus indicated on the right. (B) γδ T cells in TCRα^−/− mice. The left panels show 2-color profiles for sTCRγδ and sTCRαβ in TCRα^−/− mice with and without the Stat5b transgene. Numbers indicate the percentage of cells in each quadrant, and total cellularity is indicated above the dot plots. The right panels show the same comparison gated on CD3⁺ cells. Representative data are shown from 1 of 2 similar experiments.