A_2AR signaling promotes decreases signaling of the Ras-MAP-Kinase pathway. (A) Percentage of A_2AR Wt or KO T cells that were IFN-γ positive on rechallenge after incubation with peptide in the absence () or presence () of 1 μM CGS during induction (*P < 0.05). (B) Representative Western blots for phospho-ERK and total ERK (top and bottom, respectively). Activated CD4⁺, 6.5⁺ primary T cells were stimulated with anti-CD3⁺ anti-CD28 in the absence or presence of 1 μM CGS. A vertical line has been inserted to indicate a repositioning of gel lanes from the same experiment. (C) Representative Western blots for junB, and actin (top and bottom, respectively). CD4⁺, 6.5⁺ primary T cells were stimulated with HA and irradiated APCs overnight in the absence or presence of 1 μM CGS. (D) Representative EMSA for AP-1. CD4⁺, 6.5⁺ primary T cells were stimulated with HA and irradiated APCs overnight in the absence or presence of 1 μM CGS. Data are representative of 3 independent experiments.