Flow cytometric evaluation of 24 TCRVβ subfamilies in revertant and mutant populations. TCRVβ expression of CD18⁺ or CD18⁻ CD8⁺ T lymphocytes for each patient was evaluated by flow cytometry, analyzed using Cellquest software and summarized using bar graphs. Gating is based on CD8⁺/CD18⁺ or CD8⁺/CD18⁻ lymphocytes. CD18⁺ revertant T cells are represented by black bars; CD18⁻ T cells are represented by gray bars. TCRVβ subset was considered not expressed if it was detected in less than 0.1% of the gated population. For all 3 patients, revertant cells expressed fewer TCR Vβ subsets compared with CD18⁻ T cells. Skewing of TCVβ subfamilies is most prominent in patient 3.