Immunohistochemical examination to detect the macrophages in skin biopsies from anti-Ova injected areas of mice treated with or without FcγR dimers. (A) Skin biopsies of the above mentioned sections were processed to detect the macrophages (brown cells indicated by arrow) by immunohistochemistry. (Ai) Accumulation of macrophages in anti-Ova antibody injected area of untreated mice. (Aii) Presence of resident macrophages in PBS injected area of untreated mice. (Aiii) Inhibition of macrophage accumulation in anti-Ova antibody injected area of CD16A-Ig treated mice. (Aiv) Accumulation of macrophages in anti-Ova antibody injected area of CD32A-Ig treated mice. (B) Macrophages were counted in 10 random sites at 63x magnification and the sum of the macrophages is presented. (C) Skin biopsies of the above mentioned sections from complement-depleted mice were processed to detect the macrophages (brown cells indicated by arrow) by immunohistochemistry. (Ci) Accumulation of macrophages in anti-Ova antibody injected area of untreated mice. (Cii) Presence of resident macrophages in PBS injected area of untreated mice. (Ciii) Inhibition of macrophage accumulation in anti-Ova antibody injected area of CD16A-Ig treated mice. (Civ) Accumulation of macrophages in anti-Ova antibody injected area of CD32A-Ig treated mice. (D) Macrophages were counted in 10 random sites at 63x magnification and the sum of the macrophages is presented.