G-CSF does not stimulate the chemotaxis or chemokinesis of murine neutrophils. Freshly isolated murine bone marrow neutrophils were used in a chemotaxis assay. Neutrophils were placed on top of a chemotaxis plate in the presence or absence of G-CSF (10 nM). KC (1 nM), MIP-2 (1 nM), or G-CSF (10 nM) were added to the bottom chamber as indicated. Migration of neutrophils is expressed as the mean number of cells counted per bottom well, mean plus or minus SD. A representative figure of 3 independent experiments is shown (n = 3). ***P < .001 (1-way ANOVA and Dunnett test).