FCRL1 expression on B cells in human PBMCs and B-NHL cell lines. (A) PBMCs from a healthy donor were stained with marker mAb combination of anti–CD19-Pacific blue, anti–CD3-PerCP-Cy5.5, anti–CD14-FITC, and anti–CD56-APC, anti-FCRL1 E3-PE, or isotype IgG1-PE and analyzed with a LSRII flow cytometer. Each panel shows the overlay of cells from indicated gates stained with E3-PE (solid line) or isotype IgG-PE (gray histogram). (B) B-cell lines were stained with E3-PE (solid line) or isotype IgG1-PE (gray histogram). The MFIs subtracted by the value of isotype control are shown in parentheses.