Association of LMIR5 with adaptor molecules such as DAP10, DAP12, and FcRγ. (A,B) Ba/F3 cells were cotransduced with a Myc-tagged mLMIR5 or hLMIR5 and either a Flag-tagged DAP10, DAP12, FcRγ, or mock. (A) Cell-surface expression levels of mLMIR5 (top row) or hLMIR5 (bottom row) were analyzed by flow cytometry by staining cells with control mouse IgG1 or anti-Myc mAb, followed by FITC-conjugated anti-mouse Ig polyclonal Ab. The result of LMIR5 staining in the presence or absence of indicated adaptor molecule was represented by bold- or thin-lined histograms, respectively, while that of control staining was represented by a filled histogram. (B) Lysates of trasduced-Ba/F3 cells were immunoprecipitated with anti-mLMIR5 Ab, anti-Myc Ab, or anti-Flag mAb, and then immunoblotted with anti-Flag mAb or anti-Myc mAb. (C) Lysates of mLMIR5-transduced BMMCs were immnoprecipitated with control IgG or anti-mLMIR5 Ab, and then immunoblotted with anti-DAP12 Ab or anti-Flag mAb.