Role of PKCζ on FL response to RTX. (A) Clonogenicity was evaluated on RL cells stably transfected with pcDNA3 (1G5) or CA-PKCζ (6F9, 7E5) treated with RTX at 10, 50, or 100 μg/mL. Clonogenicity was observed after 7 days in methylcellulose medium as described in “Clonogenic assays.” Results are the mean (± SD) of 3 independent experiments. *P < .05. (B) Effect of RTX and rapamycin association was evaluated on RL cell proliferation by trypan blue exclusion assay 7 days after treatment with RTX at 10 μg/mL, rapamycin at 5 nM, or both. Results are the mean (± SD) of 3 independents experiments. *P < .05.