Role of PKCζ on mTOR inhibition induced by RTX in FL cell lines. (A) FL cell lines were treated or not with RTX at 10 and 50 μg/mL for 24 hours, and then mTOR activity was evaluated by visualizing p70S6K phosphorylation on Thr389. p70S6K was used as control of protein expression. Results are representative of 3 independent experiments. (B) The effect of PKCζ on mTOR inhibition induced by RTX was evaluated by Western blot using anti–phospho-p70S6K antibody in RL cells stably transfected with pcDNA3 (1G5) or CA-PKCζ (6F9, 7E5) treated with RTX at 50 μg/mL. p70S6K was used as control of protein expression. Results are representative of 3 independent experiments.