Expression and activity of PKCζ in NHL cell lines. PKCζ expression and activity were evaluated by Western blot using anti-PKCζ and anti–phospho-PKCζ (Thr410/403) in (A) FL (Karpas-422 and RL), DLBCL (DEAU and LIB), and MCL (Granta and Rec-1) cell lines; (B) FL cell lines compared with B lymphocytes from healthy donor (BL), B-CLL cell lines (MEC-2 and JVM-3), and B-CLL patients (patient 1 and patient 2); and (C) tissue extracts from either FL specimen or tonsils. For panels A,B, results are representative of 3 independent experiments.