Progenitor/stem cell functional assays for DMAPT treated cells. (A) AML versus normal cells were treated with 5 μM DMAPT for 18 hours in suspension culture, followed by plating in methylcellulose culture. Horizontal bars represent the mean. **P < .001 AML versus erythroid and AML versus myeloid. The percentage of colony-forming units (CFU) was normalized to untreated control. All assays were performed in triplicate. Error bars represent SEM. (B) Representative examples of the percentage of engraftment achieved in NOD/SCID mice receiving AML (left panel) or normal CB (right panel) cells after 18 hours of culture with or without 7.5 μM DMAPT. Each symbol represents a single animal analyzed at 6 to 8 weeks after transplantation. Mean engraftment is indicated by the horizontal bars. ***P < .001 DMAPT versus untreated (UNT).