Cdx2 confers self-renewal properties to murine 5-FU BM progenitors. (A) Serial replating capacity of BM progenitors constitutively expressing Cdx2, ΔN-Cdx2, or the empty control vector (EGFP). (B) Yield of cells generated in the serial replating assays of BM progenitors constitutively expressing Cdx2, ΔN-Cdx2, or the empty control vector (EGFP). (C) Morphology of colonies obtained from Cdx2-, ΔN-Cdx2–, and EGFP-expressing BM cells in replating assays. (D) Blast morphology of cells after the fourth replating (May-Grunwald-Giemsa–stained cytospin preparations). (E) Coexpression of the myeloid markers Gr1 and Mac1 on Cdx2⁺ cells obtained from the fourth round of replating. p indicates plating. Error bars indicate SD.