Granule content of peripheral T lymphocytes in control and ALPS. Splenocytes (ALPS-I in panels A and B) or freshly drawn heparinized peripheral blood samples were stained for TCRαβ, CD3, CD4, and CD8 expression. Cells were next stained for GrzA, GrzB, and perforin content. (A) Intracellular GrzA and -B content in total CD3⁺ (left), in CD3⁺/CD4⁺ (middle), or in CD3⁺/CD8⁺ (right) and (B) GrzA content in DN T cells (TCRαβ⁺ CD4⁻CD8⁻) are represented for P3 (ALPS-I), P8 (ALPS-0), and CT. Numbers on plots are percentages of total cells. (C) The percentage of GrzA⁺ and GrzB⁺ in CD4⁺ or CD8⁺ T cells from 13 ALPS-I patients and 9 aged-related healthy donors or (D) GrzA, GrzB, and perforin expression in DN T cells from 5 to 9 ALPS-I. Percentages (dots) and means (bars) are plotted. **P < .005; ***P < .001