Combined donor and recipient feeding has an additive protective effect and protection is predicted by the kinetics of Th-2 biased donor T cells in secondary lymphoid organs and the liver. (A) Survival rate of mice receiving TCD-BM alone (●, n = 10). Survival rate of recipients of TCD-BM with T cells when donor and recipient were treated with PBS (▵, n = 10) or AT (AT(D + R), ○, n = 10). Recipients of TCD-BM with T cells when the donor (□, n = 10) or the recipient (▾, n = 10) was treated with AT. Survival percentage of Balb/c recipients when both recipient and donor were fed with AT (65%), when only the T-cell/BM donors were fed with AT (43%) and when only the recipient was fed with AT (40%). (B) Serum was collected from recipients on the indicated days after BMT and IFN-γ and TNF levels were determined by ELISA (*P < .05, **P < .01). (C) Adoptively transferred CD4⁺H-2K^q+ donor T cells were isolated from the indicated organs and analyzed for their intracellular cytokine profile on days 3, 15, and 25. The y-axis depicts the percentage of IL-4–, IL-10–, TNF-, or IFN-γ–positive CD4⁺ T cells within the total CD4⁺H-2K^q+ donor population. Data presented are derived from 2 independent experiments (3 mice per time point, values are mean plus or minus standard deviation [SD]).