Competition ELISA of C2 MAbs. (A) Venn diagram showing the overlap patterns of 30 anti-C2 MAbs in a competition ELISA. Groups A, B, and C are defined based on overlaps with the basis set MAbs, ESH4, 1B5, and ESH8, respectively. (B) Competition matrix showing the interaction of the 30 anti-C2 antibodies. The rows represent primary MAbs, and the columns represent biotinylated secondary MAbs. Noncompeting and competing MAbs are shown in gray and white, respectively. MAb numbering is defined in Table 1.