TCL1 inhibits PKCθ activation. (A) Jurkat-C and Jurkat-TCL1 were stimulated with UCHT1 for different times, as indicated. Thr538-PKCθ phosphorylation (p-PKCθ, top) and TCL1 expression (bottom) were determined by Western blotting. Total PKCθ expression was used as a loading control (middle). A representative experiment of the 3 performed is shown. (B) Jurkat-C (gray line) and Jurkat-TCL1 (black line) were stimulated with PMA for 7 hours and CD69 expression was assessed by flow cytometry. A representative experiment of the 3 performed is shown. (C) Jurkat-C and Jurkat-TCL1 were stimulated with UCHT1 for 10 minutes at different concentrations. ZAP70 phosphorylation (p-ZAP70) and TCL1 expression were determined by Western blotting. β-Actin expression was used as loading control. A representative experiment of the 2 performed is shown.