Isoform-specific mFox-2A reduction inhibits exon 16 splicing switch in differentiated MELCs. (A) Screening of mFox-2A or mFox-2F isoform-specific shRNAs. mFox-2 isoform-specific shRNAs were tested in cells stably transfected with mFox-2A (lanes 1-5) or mFox-2F (lanes 6-10). The reduction of mFox-2 isoforms was analyzed by Western blot using anti-Flag antibody. β-actin was used as a loading control. (B) The reduction of endogenous mFox-2 isoform expression by isoform-specific shRNAs and the effects on exon 16 splicing. Reduction of mFox-2F isoform expression did not affect exon 16 splicing pattern in uninduced MELCs. Reduction of mFox-2A isoform expression in induced MELCs inhibited the exon 16 splicing switch.