Lytic activity of CTLs generated from patient 640. Autologous iDCs were generated from cryopreserved PBMCs and then transfected with either CsiRNA (□/○) or iPsiRNA (■/●). After the induction of maturation, these DCs were transfected with either MAGE-3 RNA (A) or tyrosinase RNA (B) and then used to stimulated autologous T cells in culture. After 1 restimulation, lytic activity was determined in duplicate against autologous DCs transfected with either MAGE-3 RNA or tyrosinase RNA, or the autologous melanoma cell line 640 alone or treated with IFN-γ for 3 days. Results are plotted as mean values (± SD); *P < .05 for the comparison between lytic activity of T cells induced by CsiRNA-transfected stimulator DCs versus iPsiRNA-transfected stimulator DCs measured against the same target.