Lentiviral expression of C/EBPα in adult and cord CD34⁺ cells. (A) Flow cytometry analysis for I antigen expression. Expression of the I antigen on the cells transduced with the virions prepared from the pLenti6-C/EBPα gene transfer vector, which harbored full C/EBPα coding cDNA, and those transduced with the virions prepared from the mock pLenti6 vector were analyzed using flow cytometry and detected with anti-I mAb (1:10 dilution). Open and shaded areas indicate cells detected with anti-I mAb and FITC-conjugated secondary antibody, and with FITC-conjugated secondary antibody only, respectively. (B) Expression profiles of IGnT transcripts. Real-time PCR was used to quantify the IGnT and β-actin transcripts. Quantities of the IGnTA, IGnTB, and IGnTC transcripts were normalized to that of β-actin transcript in each sample. Data were obtained from 3 detections; standard deviations are shown.