Association of Oct-2, Sp1, and C/EBPα transcription factors with the IGnTC gene 5′ promoter region. The association of Oct-2, Sp1, and C/EBPα transcription factors with the 5′ promoter region (−322 to +62 bp) of the IGnTC gene in K-562 cells was examined using ChIP analysis. A total of 1 × 10⁷ K-562 cells with or without sodium butyrate treatment (indicated as +SB and −, respectively) were used. The chromatin DNAs immunoprecipitated with 1:500 dilutions of anti–Oct-2, anti-Sp1, or anti-C/EBPα antibodies (Ab) and the input DNA controls were used for PCR amplification. Butyrate-treated cells were used for the no-antibody control in PCR amplification (leftmost lane).