Gcet1 expression by Western Blot. Western blot results of Gcet1 from transfected and lymphoma cell lines. The first 2 rows demonstrate the specificity of anti-Gcet1 on lysates of transfected HEK-GCET1 cell lines. A 46-kDa band specific to the Gcet1 protein was observed in GC-DLBCL (SUDHL4 and SUDHL6), FL (WSUNHL), and some other DLBCL (KARPAS 422, DB, and HT) and BL (RAJI and DAUDI) cell lines. The Gcet1 protein was not present in a subset of DLBCL (MHHPREB1, DOHH2), some BL (AKATA, RAMOS), PTL (KARPAS299, HUT78, JURKATT), myeloma (L-363, U226, KARPAS-620, LP-1), AML (HL60, KG1), or CML (K562, EM2) cell lines. The NLPHL-derived DEV cell line was strongly positive, whereas those derived from cHL (L428, KMHH2, L540, L1236, and DHMYZ) showed no specific band. The GAPDH band was used as an internal reference.