American Society of Hematology

Figure 5

$Figure 5. Endogenous relation between CEACAM1 and podoplanin expression in HDMECs. (A) These FACS scan studies show that 34% of HDMECs express podoplanin endogenously, indicating their lymphendothelial phenotype (top). Interestingly, double labeling of HDMECs with CEACAM1 and podoplanin show that CEACAM1 is detectable in both podoplanin-positive and podoplanin-negative HDMECs (bottom), but podoplanin-positive HDMECs express it in a higher amount. Endogenous expression of CEACAM1 in HDMECs increases during culture period. R1 indicates the cell fraction negative for podoplanin; and R2, the cell fraction positive for podoplanin. (B) At the culture passage 7-9 (P7-9), almost all HDMECs are positive for CEACAM1 (top) and reach the highest level of CEACAM1 expression at passage 13 (P13) while a great part of HDMECs at passage 7-9 (P7-9) is still negative for podoplanin, and almost all HDMECs are positive for podoplanin first at passage 13 (P13).$

Endogenous relation between CEACAM1 and podoplanin expression in HDMECs. (A) These FACS scan studies show that 34% of HDMECs express podoplanin endogenously, indicating their lymphendothelial phenotype (top). Interestingly, double labeling of HDMECs with CEACAM1 and podoplanin show that CEACAM1 is detectable in both podoplanin-positive and podoplanin-negative HDMECs (bottom), but podoplanin-positive HDMECs express it in a higher amount. Endogenous expression of CEACAM1 in HDMECs increases during culture period. R1 indicates the cell fraction negative for podoplanin; and R2, the cell fraction positive for podoplanin. (B) At the culture passage 7-9 (P7-9), almost all HDMECs are positive for CEACAM1 (top) and reach the highest level of CEACAM1 expression at passage 13 (P13) while a great part of HDMECs at passage 7-9 (P7-9) is still negative for podoplanin, and almost all HDMECs are positive for podoplanin first at passage 13 (P13).

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