Increased podoplanin expression in endothelial cells by CEACAM1 overexpression. (A) As demonstrated by these FACS scan studies the transfection of HDMECs for CEACAM1 (CC1) and CEACAM1/y− (CC1/y−) was efficient (top). Remarkably, there is a significantly higher amount of podoplanin in HDMECs overexpressing CEACAM1 (CC1), while no change is detectable in HDMECs transfected with empty vector (Evec) or CEACAM1/y− (CC1/y−). The level of VEGFR-3 is enhanced only by CEACAM1 overexpression (CC1). No alteration is observed in the expression level of CD31 used as control. (B) The quantification of these repeatedly reproduced data show clearly that full-length CEACAM1 induces a significant up-regulation of podoplanin but not mutated CEACAM1/y−. Error bars represent the standard deviation.