Patching experiments suggest CD81 and PrP^c can share the same microdomain. ROIs were drawn along the cell surface of each merge image, and profiles of green and red fluorescence along these ROI were constructed using Leica quantiation software. (Ai) Control cells were fixed then stained for PrP (red) and CD81 (green). (Aii) Cells underwent PrP antibody–induced patching (red) then fixed and dual stained for CD81 (green). Scale bars equal 10 μm. (Bi) Control cells were fixed then stained for CD81 (red) and PrP (green). (Bii) Cells underwent CD81 antibody-induced patching (red), then were fixed and dual stained for PrP (green). (Ci) Cells underwent PrP antibody–induced patching (red), then were fixed and dual stained for CD59 (green). (Cii) Cells underwent CD81 antibody–induced patching (red), then were fixed and then dual stained for CD59 (green). (Di) Cells underwent CD59 antibody–induced patching (red), then were fixed and dual stained for PrP (green). (Dii) Cells underwent CD59 antibody–induced patching (red), then were fixed and dual stained for CD81 (green).