Development of pDC progenitors, pDCs, and cDCs in CXCR4 conditionally deficient mice. (A,B) Flow cytometric analysis of the numbers of LSK Flt3^lo primitive hematopoietic cells, Flt3⁺ CLP, Flt3⁺ CMP pDC progenitors, and CD11c^intB220⁺PDCA-1⁺ pDCs in bone marrow (n = 3) (A); and CD11c^intB220⁺PDCA-1⁺ pDCs and CD11c^hiB220⁻ cDCs in the spleen (B) in pIpC-treated MxCre/CXCR4^f/wt mice, untreated MxCre/CXCR4^f/null mice, and pIpC-treated MxCre/CXCR4^f/null mice 3 weeks after the final pIpC treatment. (C) Bone marrow cells (Ly5.2⁺) from MxCre/CXCR4^f/null or MxCre/CXCR4^f/wt mice were transferred along with wild-type bone marrow cells (Ly5.1⁺) into lethally irradiated Ly5.1⁺ recipients. Chimeric mice were treated with pIpC at 13 weeks after transfer, and their bone marrow was analyzed by flow cytometry at 10 weeks after the treatment. The numbers of Ly5.2⁺ and Ly5.1⁺ CD11c^intB220⁺PDCA-1⁺ pDCs are shown (n = 2). Error bars represent SD of the mean.