American Society of Hematology

Figure 2

$Figure 2. Dramatic reduction of cells in Flt3+ subsets of CLP and CMP fractions and DCps in the absence of CXCR4. E16.5 fetal liver cells (Ly5.2+/Ly5.1−) from CXCR4+/− and CXCR4−/− mice were transferred into lethally irradiated Ly5.2−/Ly5.1+ recipients. Flow cytometric analysis of the numbers of donor-derived Ly5.2+Lin−Sca-1+c-kit+ (LSK) Flt3lo primitive hematopoietic cells (A); progenitors of pDCs, including Ly5.2+Lin−IL-7R+Sca-1loc-kit+Flt3+ (Flt3+ CLP) and Ly5.2+Lin−Sca-1−c-kit+CD34+Flt3+ (Flt3+ CMP) cells; (B) and Ly5.2+CD11c+MHC II−CD4−CD8−CD19−NK1.1− DCp's (C) in the bone marrow of CXCR4+/− and CXCR4−/− chimeric mice 16 to 45 weeks after transfer (n = 3,5). Error bars represent SD of the mean.$

Dramatic reduction of cells in Flt3⁺ subsets of CLP and CMP fractions and DCps in the absence of CXCR4. E16.5 fetal liver cells (Ly5.2⁺/Ly5.1⁻) from CXCR4^+/− and CXCR4^−/− mice were transferred into lethally irradiated Ly5.2⁻/Ly5.1⁺ recipients. Flow cytometric analysis of the numbers of donor-derived Ly5.2⁺Lin⁻Sca-1⁺c-kit⁺ (LSK) Flt3^lo primitive hematopoietic cells (A); progenitors of pDCs, including Ly5.2⁺Lin⁻IL-7R⁺Sca-1^loc-kit⁺Flt3⁺ (Flt3⁺ CLP) and Ly5.2⁺Lin⁻Sca-1⁻c-kit⁺CD34⁺Flt3⁺ (Flt3⁺ CMP) cells; (B) and Ly5.2⁺CD11c⁺MHC II⁻CD4⁻CD8⁻CD19⁻NK1.1⁻ DCp's (C) in the bone marrow of CXCR4^+/− and CXCR4^−/− chimeric mice 16 to 45 weeks after transfer (n = 3,5). Error bars represent SD of the mean.

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