LIF, IL-6, and M-CSF depletion prevents M2d generation. (A,C) Monocytes were cultured for 5 days in CM with ascites (A) or A172 SNs (C) either concomitantly depleted or not (control mAbs) of LIF, IL-6, OSM, and/or M-CSF. After 48 hours of LPS stimulation, IL-12 production and CD86 expression were analyzed. Results are expressed in percentages of restoration as means plus or minus SD; n = 6 (in panel A, experiments were performed with 6 ascites). (B,D,E) Monocytes were cultured for 5 days in CM without or with ascites (B), HepG2 SNs (D), or 5637 SNs (E) depleted or not. CD14 expression was analyzed by FACS on day 5. IL-10 and IL-12 production and ILT3 and CD86 expression were analyzed after 48 hours of LPS stimulation. (B,D,E) Left panels represent markers whose expressions are inhibited compared with M2d's; results are expressed in percentages of inhibition of marker expression. Right panels represent markers whose expressions are increased compared with M2d's; results are expressed in percentages of restoration of marker expression. (B,D,E) Results are expressed as means plus or minus SD; n = 6 (in panel B, experiments were performed with 6 ascites). *P < .05.