TAMs, TA-MΦs, LIF-MΦs, and IL-6-MΦs differ from M2a-c. Monocytes cultured for 5 days in CM were not (MΦs) or were exposed for 48 hours to IL-4 (M2a's), IL-1β (M2b's), or IL-10 (M2c's) or were cultured for 5 days in CM supplemented with ascites (TA-MΦs), LIF (LIF-MΦs), or IL-6 (IL-6-MΦs). CD86 (A), CD163 (G), CD14 (I), ILT2 (J), and ILT3 (K) expression was analyzed by FACS prior to (G,I) or after (A,J,K) 48 hours of LPS stimulation. CCL17 (B), CCL22 (C), CCL1 (D), TNFα (E), CCL18 (F), and PTX3 (H) were quantified by ELISA in the SNs of cells either unstimulated (B,C) or stimulated for 48 hours with LPS (D-F,H). Results are expressed in MFI (A,G,I-K) or in ng/mL (B-F,H). (A-K) Results are expressed as means plus or minus SD of data obtained with monocytes from 4 subjects (MΦs, M2a-c, LIF-MΦs, and IL-6-MΦs) or with 6 ovarian cancer patients (for TAMs and TA-MΦs). *P < .05 (considered significantly different from results obtained for MΦs).