Effects of APC depletion or anti-FcγRI mAb on β₂GPI/PS-induced T-cell response. (A) CD14⁺ monocyte-depleted, CD19⁺ B-cell–depleted, and mock-treated PBMCs derived from APS3 were cultured for 7 days with or without β₂GPI, PS, or β₂GPI/PS in medium supplemented with autologous APS plasma, and the T-cell proliferative response was measured by ³H-thymidine incorporation. Results are shown as mean (column) and standard deviation (error bar) of duplicate measurements. Analogous results were obtained in a total of 4 independent experiments using samples from 3 patients with APS (APS1, APS3, and APS4). (B) PBMCs from APS2 were cultured for 7 days with or without β₂GPI, PS, or β₂GPI/PS in medium supplemented with autologous APS plasma. Anti-FcγRI or isotype-matched control mAb was added to the initiation of cultures. The T-cell proliferative response was evaluated by ³H-thymidine incorporation. Results are shown as mean (column) and standard deviation (error bar) of duplicate measurements. Concordant results were obtained with samples from 3 patients with APS (APS2, APS3, and APS9).