Figure 2
Figure 2. Proliferative responses of β2GPI/PS-primed T cells to various β2GPI preparations in secondary cultures. PBMCs from APS3 (top) and APS4 (bottom) were stimulated with β2GPI/PS for 10 days in medium supplemented with autologous plasma. The viable T cells were then cultured in duplicate with β2GPI, reduced β2GPI, GP-F, GP1, GP2, GP3, MalBP, p276-290, or p306-320 in medium containing FBS. After 3 days, 3H-thymidine incorporation was measured. Results are shown as mean (column) and standard deviation (error bar) of duplicate measurements.

Proliferative responses of β2GPI/PS-primed T cells to various β2GPI preparations in secondary cultures. PBMCs from APS3 (top) and APS4 (bottom) were stimulated with β2GPI/PS for 10 days in medium supplemented with autologous plasma. The viable T cells were then cultured in duplicate with β2GPI, reduced β2GPI, GP-F, GP1, GP2, GP3, MalBP, p276-290, or p306-320 in medium containing FBS. After 3 days, 3H-thymidine incorporation was measured. Results are shown as mean (column) and standard deviation (error bar) of duplicate measurements.

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