The effect of kinase inhibitors on potentiation of 2-MeSADP–induced platelet aggregation by IGF-1. (A) Washed platelets preincubated with 100 nM wortmannin, 10 μM dimethyl-BAPTA, or 10 μM GF109203X were stimulated at 37°C with either 30 nM 2-MeSADP or a combination of 30 nM 2-MeSADP and 100 nM IGF-1, as noted. (B) Washed platelets preincubated with 100 nM wortmannin, 500 nM PIK-75, 500 nM TGX-221, 2 μM AS-252424, or 1 μM IC87114 were stimulated at 37°C with either 30 nM 2-MeSADP or a combination of 30 nM 2-MeSADP and 100 nM IGF-1, as noted. All aggregations were performed in the presence of 1 mg/mL fibrinogen. Tracings are representative of results obtained from 3 different donors. (C) The percentage of potentiation of 2-MeSADP–induced platelet aggregation by IGF-1 in the presence of specific PI3-K inhibitors shown in panel B is quantified in the bar graphs, and the results are means plus or minus SE (n = 3).