Figure 1
![Figure 1. TDZD-8 specifically induces cell death of primary leukemia specimens. (A) Primary AML (n = 37), CLL (n = 12), ALL (n = 6), bcCML (n = 6), and normal mononuclear cells (n = 13, obtained from BM [n = 3], CB [n = 7], or MPB [n = 3]) were cultured for 18 to 24 hours in the presence of 20 μM TDZD-8. Cell viability was assessed by Annexin V/7-AAD staining. Percent viability is represented relative to untreated control. Leukemia specimens were significantly (P < .001) more sensitive to TDZD-8 than were normal specimens. Error bars represent the SEM. All assays were performed in triplicate. (B) Representative dot plots for the 24-hour flow cytometric analysis with Annexin V/7-AAD stain in the presence or the absence of 20 μM TDZD-8.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/13/10.1182_blood-2007-05-088815/3/zh80010810670001.jpeg?Expires=1722357086&Signature=qUdpI1~1Az8QKcKy~JHq2kGldJlSY9lTDMlQw8G8lp5Poh01d~Bol9Xrq8Dt2qpkaYAsbcVqWfXDZsilHX3Rc2fuo6MirsDbN-qRFiioBEkblZUlE9p7LpGc4yJNtS8oIKEWyqndtzWZI2HHj-oeXcSMiW63H6Kpuvr3bC33B0Zt~j5XWzzzzcy~EsWG~5XAbG3HtbvquNDFT7B83VbZWokuwfNth-CtLUQwKy2tGd6Q422Q9Jb1cll4MExx6UtbHLJiU76AXzQbP5WNO-A6RdLLVguCUf4YAc2jcwq5NzQRsBGaZnBizArSXJFAHhbcUtL13OBiC8kYiz48FLN-rg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TDZD-8 specifically induces cell death of primary leukemia specimens. (A) Primary AML (n = 37), CLL (n = 12), ALL (n = 6), bcCML (n = 6), and normal mononuclear cells (n = 13, obtained from BM [n = 3], CB [n = 7], or MPB [n = 3]) were cultured for 18 to 24 hours in the presence of 20 μM TDZD-8. Cell viability was assessed by Annexin V/7-AAD staining. Percent viability is represented relative to untreated control. Leukemia specimens were significantly (P < .001) more sensitive to TDZD-8 than were normal specimens. Error bars represent the SEM. All assays were performed in triplicate. (B) Representative dot plots for the 24-hour flow cytometric analysis with Annexin V/7-AAD stain in the presence or the absence of 20 μM TDZD-8.
