Exosomal miR-214 induces migration and angiogenesis in vitro. Bioanalyzer profiles of small RNA from (A) cells and (B) exosomes are shown with miRNA indicated. (C) qPCR analysis of miR-214 in endothelial cells and exosomes is shown relative to RNU19 (relative to cell content, n = 6 ± SD, Student t test). (D) miR-214 analysis on RNA from sucrose density gradient fractions is shown. Ct values were subtracted from the fraction showing the highest Ct value (fraction a: Ct = 39.49; B, bottom fraction). (E) Western blot detected flotillin-1 in the corresponding sucrose density gradient fractions; B, bottom fraction. (F) This schematic overview shows the procedure for generating anti–miR-214 and control miR-214 cells and exosomes. The graph shows (G) qPCR analysis of miR-214 content in anti–miR-214 and control-miR-214 cells and exosomes. (Values are plotted relative to samples from cells that were transfected with NT negative control RNA (n = 4 ± SD, Student t test). Quantification of (H) migration (n = 5, ± SD, Student t test), (I) angiogenesis (n = 5, ± SD, Student t test), and (J) sprouting assays (n = 3, ± SD, Student t test) in which endothelial cells were treated with anti–miR-214 or control miR-214 exosomes.