Relative miRNA levels in endothelial cells cocultured with platelets. Levels of miR-22, -185, -320b, and 423-5p in HMEC-1 cells cocultured with platelets in the presence or absence of 1 U/mL thrombin at indicated time points. Levels are expressed relative to miR-16 and normalized to the mean baseline expression in each group (n = 3). Two-way analysis of variance with Bonferroni post hoc test was used to assess the effect of platelet activation on the levels of miRNA in HMEC-1 cells (*P < .05; **P < .01; ***P < .001).