Validation of differentially expressed genes by quantitative PCR. The relative expression of Prl2a1, Il10, Sox4, Rgs13, and Sell in whole-spleen tissue of 36-week-old mice (n = 3/genotype) was measured by real-time quantitative PCR. Data are presented as mean fold change relative to WT mice; error bars represent the SEM. Significant differences between genotypes are indicated (*P < .05; **P < .01; ***P < .001).