Vitamin A deprivation in recipient mice impairs the acquisition of gut-homing molecule expression on donor T cells after BMT. Lethally irradiated Balb/c Rag mice fed with either VAS or VAD diets were transplanted with B6 BM and CellTrace-violet–labeled splenic T cells (adjusted to yield a T-cell dose of 1 × 10⁶). Mice from both groups were killed on day 3 after BMT. (A) Representative contour plots of CCR9 and α4β7 expression on donor T cells isolated from the MLNs of recipient mice after gating on H-2K^b+ CD4⁺ or H-2K^b+ CD8⁺ cells. (B) The mean percentage positive donor T cells (± SEM) expressing CCR9 and α4β7 from 4 mice per group. Data are from 1 of 2 representative experiments. Statistics: ***P ≤ .001. (C) Representative contour plots of CCR10 expression on donor T cells isolated from the PLNs of recipient mice. (D) The mean percentage positive donor T cells (± SEM) expressing CCR10 from 4 mice per group. Data are from 1 of 2 representative experiments. Statistics: *P ≤ .05, **P < .01. (E) Representative example of CellTrace-violet-labeled donor H-2K^b+ CD4⁺ or CD8⁺ T-cell proliferation in the spleen of VAS and VAD recipients 3 days after transplantation. (F) Mean (± SEM) percentage of proliferating (CellTrace-violet low) splenic donor T cells (n = 4 mice per group). One of 2 representative experiments is shown.