Phylogenetic trees of leukemia single cells at diagnosis and relapse from 2 AML patients. a, L1: Left – reconstructed lineage tree of CD33+ CD4+ CD15- peripheral blood (PB) individual leukemia cells at diagnosis (full, n=14); and relapse (blank, n=27) for patient L1; The root was determined by calculation of the median signal of all cells. Right - comparison of median depth between cells at diagnosis (full) and relapse (blank), (p<10^^-5). b, L2: Left – reconstructed lineage tree of CD117+ PB individual leukemia blast cells at diagnosis (full, n=33), LSCs LIN-CD34+ CD38- CD90+ (gray, n=21) and relapse(blank, n=10) for patient L2. Right - Comparison of median depth between cells at diagnosis (full) and relapse (blank), (p=0.0001). Y axis represents depth in arbitrary units (logarithmic scale).