Figure 1.
Figure 1. Upregulation of CD107a expression by anti-CD70 antibody on HTLV-1 Tax tetramer specific cytotoxic T lymphocytes (CTLs). (a) Representative flow cytometric dot plots showing increased CD107a expression in HTLV-1 Tax tetramer specific CTLs treated with anti-CD70 blocking antibody compared with isotype antibody. Freshly isolated peripheral blood mononuclear cells were incubated with or without 0.02 μM HTLV-1 Tax peptide in the presence of anti-CD70 blocking antibody or isotype control (10 μg/ml) for 4 hours. Analysis was performed on a FACScan flow cytometer (BD Bioscience, San Jose, CA, USA) and with FlowJo software (Tree Star, Ashland, OR, USA). Lymphocytes were gated on forward and side light scatter properties, followed by gating on CD8+ tetramer+ CTLs. (b) Summary of CD107a expression in HTLV-1 Tax tetramer specific CTLs after incubation with anti-CD70 blocking or isotype control antibody. P < 0.05, Wilcoxon matched-pairs test.

Upregulation of CD107a expression by anti-CD70 antibody on HTLV-1 Tax tetramer specific cytotoxic T lymphocytes (CTLs). (a) Representative flow cytometric dot plots showing increased CD107a expression in HTLV-1 Tax tetramer specific CTLs treated with anti-CD70 blocking antibody compared with isotype antibody. Freshly isolated peripheral blood mononuclear cells were incubated with or without 0.02 μM HTLV-1 Tax peptide in the presence of anti-CD70 blocking antibody or isotype control (10 μg/ml) for 4 hours. Analysis was performed on a FACScan flow cytometer (BD Bioscience, San Jose, CA, USA) and with FlowJo software (Tree Star, Ashland, OR, USA). Lymphocytes were gated on forward and side light scatter properties, followed by gating on CD8+ tetramer+ CTLs. (b) Summary of CD107a expression in HTLV-1 Tax tetramer specific CTLs after incubation with anti-CD70 blocking or isotype control antibody. P < 0.05, Wilcoxon matched-pairs test.

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