Deptor is a direct downstream target of NOTCH1 signaling. Compound E treatments decreased the mRNA (A) and protein expression (B) of deptor. (C) Analyses of 174 T-ALL patients revealed that mRNA expression of deptor is highly correlated with notch1 and hes1. (D) The schematic presentation of deptor promoter. Two CSL binding site sequences are shown. TIS, transcription initiation site. (E) ChIP assays revealed that NOTCH1 directly bound to the deptor promoter and Compound E treatments abolished the association. hes1 and actin promoters are shown as positive and negative controls.